Phosphatidylinositol 4-Kinase Type IIα Is Targeted Specifically to Cellugyrin-Positive Glucose Transporter 4 Vesicles
نویسندگان
چکیده
منابع مشابه
Phosphatidylinositol 4-kinase is a component of glucose transporter (GLUT 4)-containing vesicles.
We recently developed a procedure for immunoisolating insulin-responsive membrane vesicles that contain the muscle/fat glucose transporter isoform, GLUT 4, from rat adipocytes. Utilizing this methodology, we are analyzing the components of these vesicles to gain an understanding of how they are regulated by insulin. In this report we identify a phosphatidylinositol (PtdIns) 4-kinase as a consti...
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Phosphorylation of inositol phospholipids plays a key role in cellular regulation via the generation of intracellular second messengers. In addition, it represents a mechanism to regulate interactions of the lipid bilayer with proteins and protein scaffolds involved in vesicle budding, cytoskeletal organization, and signaling. Generation of phosphatidylinositol 4-phosphate [PI(4)P] from phospha...
متن کاملMolecular insights into the membrane-associated phosphatidylinositol 4-kinase IIα
Phosphatidylinositol 4-kinase IIα (PI4KIIα), a membrane-associated PI kinase, plays a central role in cell signalling and trafficking. Its kinase activity critically depends on palmitoylation of its cysteine-rich motif (-CCPCC-) and is modulated by the membrane environment. Lack of atomic structure impairs our understanding of the mechanism regulating kinase activity. Here we present the crysta...
متن کاملThe crystal structure of the phosphatidylinositol 4-kinase IIα.
Phosphoinositides are a class of phospholipids generated by the action of phosphoinositide kinases with key regulatory functions in eukaryotic cells. Here, we present the atomic structure of phosphatidylinositol 4-kinase type IIα (PI4K IIα), in complex with ATP solved by X-ray crystallography at 2.8 Å resolution. The structure revealed a non-typical kinase fold that could be divided into N- and...
متن کاملPhosphatidylinositol 4-kinase IIα function at endosomes is regulated by the ubiquitin ligase Itch.
Phosphatidylinositol (PI) 4-phosphate (PI(4)P) and its metabolizing enzymes serve important functions in cell signalling and membrane traffic. PI 4-kinase type IIα (PI4KIIα) regulates Wnt signalling, endosomal sorting of signalling receptors, and promotes adaptor protein recruitment to endosomes and the trans-Golgi network. Here we identify the E3 ubiquitin ligase Itch as binding partner and re...
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ژورنال
عنوان ژورنال: Molecular Endocrinology
سال: 2006
ISSN: 0888-8809,1944-9917
DOI: 10.1210/me.2006-0193